Mouse Vaccinia Virus B5R Monoclonal Antibody, 0.1 mg

Overview of Mouse Vaccinia Virus B5R Monoclonal Antibody

The Mouse Vaccinia Virus B5R Monoclonal Antibody is a highly specific reagent designed for the detection and analysis of the B5R protein in Vaccinia virus. Vaccinia virus, a member of the Orthopoxvirus genus, serves as a model for the study of related poxviruses, including variola (smallpox) and monkeypox. The B5R gene encodes a protein crucial for the virus's ability to infect and spread within the host.

Structure and Function of B5R Protein

The B5R protein is a membrane-associated glycoprotein that plays a critical role in the formation of viral extracellular enveloped virions (EEVs), which are essential for the virus's ability to disseminate through the host. It is involved in the fusion of viral and host cell membranes, facilitating the release of viral particles from infected cells. The B5R protein is also important for immune evasion, as it can inhibit host immune responses by modulating cellular signaling pathways.

Antibody Specificity

This monoclonal antibody is generated by immunizing mice with a recombinant form of the Vaccinia B5R protein. The resulting monoclonal antibody binds specifically to the B5R glycoprotein, making it an invaluable tool for studying the expression, localization, and functional role of the protein within the context of virus-host interactions. The antibody can be used in a variety of immunological applications, including:

• Western blotting: For detection of B5R protein expression in infected cells or tissues.
• Immunofluorescence: To visualize the subcellular localization of B5R in infected cells.
• ELISA: For quantitative measurement of B5R protein levels in culture supernatants or other biological samples.

Applications in Research

The Mouse Vaccinia Virus B5R Monoclonal Antibody is primarily used in virology and immunology research, particularly in studies focused on the biology of Vaccinia virus and its pathogenesis. It facilitates the investigation of viral replication cycles, viral entry mechanisms, and interactions between the virus and host immune cells. Additionally, it is employed in research into viral vaccine development, as understanding the function of proteins like B5R can inform the design of better therapeutic and preventive measures against poxvirus infections.

Conclusion

In summary, the Mouse Vaccinia Virus B5R Monoclonal Antibody is a specialized tool for the detection and study of the B5R glycoprotein in Vaccinia virus, offering significant utility in both basic and applied virology research. It supports investigations into viral pathogenesis, immune response evasion, and the development of poxvirus-based vaccines.